The Great Capacity on Promoting Melanogenesis of Three Compatible Components in Vernonia anthelmintica (L.) Willd.

To investigate a possible methodology of exploiting herbal medicine and design polytherapy for the treatment skin depigmentation disorder, we have made use Vernonia anthelmintica (L.) Willd., traditional Chinese that has been proven to be effective in treating vitiligo. Here, report extract Willd. effectively enhances melanogenesis responses B16F10. In its compound library, found three ingredients (butin, caffeic acid luteolin) also activity promoting vivo vitro. They can reduce accumulation ROS induced by hydrogen peroxide inflammatory response sublethal concentrations copper sulfate wild type green fluorescent protein (GFP)-labeled leukocytes zebrafish larvae. The overall objective present study aims identify which compatibility proportions medicines may more pigmentation. We utilized D-optimal surface optimize ratio among molecules. Combining indicators melanogenesis, anti-inflammatory antioxidant capacities, get best effect butin, luteolin at (butin:caffeic acid:luteolin = 7.38:28.30:64.32) on zebrafish. Moreover, melanin content recovery combination is stronger than monomer, suggests compounds synergistic inducing melanogenesis. After simply verifying result, performed situ hybridization whole-mount embryos further explore effects multi-drugs proliferation differentiation melanocytes expression genes (tyr, mitfa, dct, kit) related synthesis. conclusion, above compatible significantly enhance improve vivo.